Gene expression after SPVD infection in resistant and susceptible sweet potato cultivars

Sweet potato virus disease (SPVD) is one of the most devastating diseases affecting sweetpotato (Ipomoea batatas), an important food crop in developing countries. SPVD develops when sweetpotato plants are dually infected with sweet potato feathery mottle virus (SPFMV) and sweet potato chlorotic stunt virus (SPCSV). In the current study, global gene expression between SPVD affected plants and virus-tested control plants (VT) were compared in the susceptible ‘Beauregard’ and resistant ‘NASPOT 1’ (Nas) sweetpotato cultivars at 5, 9, 13 and 17 days post inoculation (DPI). Two-color (Cy3 and Cy5) microarrays. 2 cultivars: Beauregard (Bx) and Naspot 1 (Nas); 4 time points: 5dpi, 9dpi, 13 dpi, 17 dpi; Treatments : SPVD and Virus Tested Control; 4 replicates (plants) for each cultivar x treatment combination. Please note that the loop design used for this study necessitated the use of 2 "samples" for each array. So there are 64 sample entries and 32 arrays. Use the title of the supplementary file to determine which samples were hybridized on the same array. For example GSM467650_BNHS1_1.gpr.gz and GSM467651_BNHS1_2.gpr.gz indicated that GSM467650 and GSM467651 were hybridized on the same array (Lab array # BNHS1).

甘薯病毒病（Sweet potato virus disease, SPVD）是危害甘薯（Ipomoea batatas）的最具破坏性的病害之一，而甘薯是发展中国家的重要粮食作物。当甘薯植株同时感染甘薯羽状斑驳病毒（sweet potato feathery mottle virus, SPFMV）与甘薯褪绿矮化病毒（sweet potato chlorotic stunt virus, SPCSV）时，便会引发SPVD。本研究分别于接种后5、9、13和17天（days post inoculation, DPI，后文简称dpi），对易感品种‘Beauregard’与抗病品种‘NASPOT 1’（简称Nas）中感染SPVD的植株与病毒检测对照植株（virus-tested control plants, VT）的全局基因表达水平进行了比较。实验采用双色（Cy3与Cy5）微阵列技术，实验设置如下：2个甘薯品种：Beauregard（简称Bx）与Naspot 1（简称Nas）；4个时间点：5dpi、9dpi、13dpi、17dpi；处理分组：SPVD感染组与病毒检测对照组；每个品种-处理组合设置4次植株生物学重复。需注意，本研究采用的环路设计要求每张芯片需使用2份样本进行杂交，因此本数据集共包含64个样本条目与32张芯片。可通过补充文件的标题判断同一张芯片上的杂交样本：例如GSM467650_BNHS1_1.gpr.gz与GSM467651_BNHS1_2.gpr.gz表明，GSM467650与GSM467651在同一张芯片（实验室芯片编号BNHS1）上完成杂交。