Oligonucleotides inserted into the BbsI site of pSpCas9(BB)-2A-GFP.

sgRNA sequences are indicated in bold and preceded by a G nucleotide to improve transcription [29].单引导RNA（single guide RNA，sgRNA）序列以粗体标注，且在其前添加一个G核苷酸以提升转录效率[29]。