The role of Stk in Streptococcus pyogenes

Transcriptional profile of Streptococcus pyogenes stk mutant strain JRS2516 vs its wild type parent strain MGAS2221 The RNA prepared from triplicate cultures for the wild type and the stk mutant strains was each labeled with Cy3 and hybridized to duplicate arrays. Reference RNA consisted of pooled RNA for the wild type and stk mutant, labeled with Cy5.

化脓性链球菌（Streptococcus pyogenes）stk突变菌株JRS2516与其野生型亲本菌株MGAS2221的转录组谱
分别从野生型菌株与stk突变菌株的三份生物学重复培养物中提取RNA，将每份RNA均以Cy3标记后与两份重复的基因芯片阵列进行杂交。对照RNA由野生型菌株与stk突变菌株的混合RNA组成，并以Cy5标记。