Bulk RNA-seq of adult pig and rat central canal–associated spinal cord cells

Bulk RNA sequencing was performed on central canal–associated spinal cord cells isolated from adult pig (Sus scrofa) and rat (Rattus norvegicus) spinal cords and expanded under ex vivo culture conditions. Libraries were sequenced on the Illumina NovaSeq 6000 platform using 150 bp single-end reads. Transcript abundances were quantified using Salmon (v1.x) against species-specific reference transcriptomes (pig: ss11; rat: rn6). This dataset enables cross-species analysis of transcriptional programs associated with proliferative activation and progenitor-associated states in the mammalian spinal cord. Overall design: Central canal–associated regions were microdissected from adult pig and rat spinal cords. Cells were expanded as adherent cultures under defined serum-free conditions. Total RNA was extracted and strand-specific poly(A)+ libraries were prepared using the Illumina TruSeq Stranded mRNA workflow. Libraries were sequenced on an Illumina NovaSeq 6000 (150 bp, single-end). Transcript quantification was performed with Salmon (v1.x) using ENSEMBL/ENCODE v104 transcript annotations. Raw FASTQ files and per-sample transcript-level quantifications (quant.sf) are provided.

本数据集针对从成年猪（家猪，*Sus scrofa*）和大鼠（褐家鼠，*Rattus norvegicus*）脊髓中分离并经体外（ex vivo）培养扩增的中央管相关脊髓细胞开展了批量RNA测序（Bulk RNA sequencing）。测序文库在Illumina NovaSeq 6000测序平台上以150 bp单端读长进行测序。转录本丰度使用Salmon（v1.x），针对物种特异性参考转录组进行定量，其中猪参考转录组版本为ss11，大鼠为rn6。本数据集可用于开展哺乳动物脊髓中与增殖激活及祖细胞相关状态相关的转录程序的跨物种分析。

整体实验设计：
从成年猪和大鼠脊髓中显微切割获取中央管相关区域；将细胞在限定的无血清培养条件下以贴壁培养方式进行扩增。提取总RNA，并采用Illumina TruSeq Stranded mRNA建库流程制备链特异性poly(A)+富集文库。测序文库在Illumina NovaSeq 6000平台上以150 bp单端读长完成测序。转录本定量采用Salmon（v1.x），并使用ENSEMBL/ENCODE v104版本的转录本注释信息。本数据集提供原始FASTQ文件及每个样本的转录本水平定量结果（quant.sf）。