Single-cell transcriptomic analysis of dermal macrophage from PDGFRa-lineage origin under atopic dermatitis

We analyzed dermal macrophage repertoire derived from PDGFRa-lineage or non-PDGFRa-lineage origin in atopic dermatitis (AD) with single-cell RNA sequencing. PDGFRa-lineage tracing mice aged between 7- and 14-week old were used to label cells of PDGFRa-lineage with tdTomato. AD was induced by topic application of MC903 bidaily or tridaily to skin of female mice for six times. Inflamed skin was excised on day 14 and single cells were prepared. Target cells were sorted as single cells for sequencing. We identified multiple subtypes in both PDGFRa-lineage or non-PDGFRa-lineage macrophage and the proportion differed by PDGFRa-lineage origin in AD skin. Transcriptomic profiling of F4/80+ MHCII+ dermal macrophage of different PDGFa-lineage origin from AD skin

本研究采用单细胞RNA测序（single-cell RNA sequencing），分析了特应性皮炎（atopic dermatitis, AD）模型中源自PDGFRa谱系（PDGFRa-lineage）或非PDGFRa谱系的真皮巨噬细胞库。实验选用7~14周龄的PDGFRa谱系示踪小鼠，通过tdTomato标记PDGFRa谱系细胞。以每日两次或三次的频率向雌性小鼠皮肤局部涂抹MC903，连续给药6次以诱导特应性皮炎模型。于造模第14天切除炎症皮肤并制备单细胞悬液，分选目标单细胞用于后续测序。本研究在PDGFRa谱系及非PDGFRa谱系的巨噬细胞中均鉴定出多种亚型，且AD皮肤中不同PDGFRa谱系来源的巨噬细胞比例存在显著差异。本研究针对特应性皮炎皮肤中不同PDGFRa谱系来源的F4/80+ MHCII+ 真皮巨噬细胞开展了转录组分析。