To examine the expression of Sulf1 and Sulf2, as well as other glycan-related genes, in human Idiopathic pulmonary fibrosis (IPF) lungs compared to normal lung samples. Homo sapiens

Microarray analysis to examine glycan-related gene expression in idiopathic pulmonary fibrosis Heparan sulfate 6-O-endosulfatases (Sulf1 and Sulf2) remove 6-O sulfate groups from heparan sulfate intra-chain sites on the cell surface and in the extracellular matrix, and modulate the functions of many growth factors and morphogens including FGF, Wnt and TGF-beta. Works from our laboratory have shown that TGF-beta 1 induces Sulf1 and Sulf2 expression in a cell-type specific manner in the lung, specifically Sulf1 in lung fibroblasts and Sulf2 in type II alveolar epithelial cells. Interestingly TGF-beta 1-induced Sulf1 and Sulf2 in turn modulate TGF-beta 1 function in culture. Overall design: The aim of this study is to examine the expression of Sulf1 and Sulf2 as well as other glycan-related genes (heparan biosynthetic enzymes, TGF-beta, FGF and Wnt signaling pathway components) in human idiopathic pulmonary fibrosis (IPF) lungs compared to normal lung samples. We will examine gene expression in triplicate samples from RNA of total lung homogenates from IPF and control (normal) lungs

本数据集采用微阵列分析技术，旨在检测特发性肺纤维化（idiopathic pulmonary fibrosis, IPF）组织中聚糖相关基因的表达水平。硫酸乙酰肝素6-O-内切硫酸酯酶（heparan sulfate 6-O-endosulfatases，Sulf1与Sulf2）可从细胞表面及细胞外基质（extracellular matrix）的硫酸乙酰肝素链内位点移除6-O硫酸基团，并调控包括成纤维细胞生长因子（fibroblast growth factor, FGF）、Wnt家族蛋白以及转化生长因子β（transforming growth factor-β, TGF-β）在内的多种生长因子与形态发生因子的功能。本实验室既往研究显示，转化生长因子β1（transforming growth factor-β1, TGF-β1）可通过细胞类型特异性的方式在肺组织中诱导Sulf1与Sulf2的表达：具体而言，肺成纤维细胞中表达Sulf1，肺泡Ⅱ型上皮细胞中表达Sulf2。有趣的是，经TGF-β1诱导产生的Sulf1与Sulf2可在体外培养体系中反向调控TGF-β1的功能。【实验整体设计】本研究的核心目标为对比人类特发性肺纤维化（IPF）肺组织与正常肺组织样本中Sulf1、Sulf2及其他聚糖相关基因（包括硫酸乙酰肝素生物合成酶、TGF-β、FGF及Wnt信号通路组分）的表达谱。我们将对特发性肺纤维化组与对照组（正常肺组织）的全肺匀浆RNA样品进行三次重复检测。