Effect of manure amendment and antibiotic treatment on the invasion potential of an antibiotic degradant and resistant strain in soil bacterial communities

The antibiotic degradation function might confer a selective advantage on antibiotic-resistant bacterial strains, thus promoting their dispersion in polluted environments. This project tested, through a microcosm study, the impact of manure amendment and sulfamethazine pollution on the invasion potential of a sulfamethazine degradant and resistant strain (Microbacterium sp. C448) in bacterial communities of four soils. Studying the soil microbial community structure and diversity by Illumina 16S rDNA sequencing helps identify the reasons for the success or failure of the strain invasion.

抗生素降解功能可赋予抗药性细菌菌株选择优势，进而促进其在受污染环境中的扩散。本项目通过微宇宙实验（microcosm study），探究了粪肥改良与磺胺二甲嘧啶污染对4种土壤细菌群落中磺胺二甲嘧啶降解型抗药菌株（Microbacterium sp. C448）的入侵潜力的影响。采用Illumina 16S rDNA测序（Illumina 16S rDNA sequencing）分析土壤微生物群落结构与多样性，有助于明确该菌株入侵成败的原因。