HDAC3 ChIP sequencing of L4-L6 Dorsal Root Ganglia upon spinal or sciatic axonal injury

Purpose: A) compare the response to an axonal injury in the peripheral vs central nervous system B) clarify the role of HDAC3 in axonal regeneration Results: we found that after peripheral axonal injury there is a higher number of differentially expressed genes regulated by HDAC3 with respect to central injury with a number of genes associated with regenerative pathways Overall design: Method:HDAC3 ChIP-seq from sciatic L4-L6 DRG tissue 24 hours after the following treatments: a) spinal cord dorsal column injury b) laminectomy c) sciatic nerve injury d) sham. Pool of sciatic DRGs from 20 mice were used for each sample. DRG were collected and flash frozen.研究目的：A) 比较外周神经系统与中枢神经系统对轴突损伤的应答差异；B) 阐明组蛋白去乙酰化酶3（HDAC3）在轴突再生过程中的调控作用。 研究结果：本研究发现，相较于中枢神经系统轴突损伤，外周轴突损伤后，受HDAC3调控的差异表达基因数量更多，且其中存在大量与轴突再生通路相关的基因。 实验设计与方法：对接受以下4种处理后24小时的坐骨神经L4-L6节段背根神经节（Dorsal Root Ganglion, DRG）组织进行HDAC3染色质免疫共沉淀测序（ChIP-seq）：a) 脊髓背柱损伤；b) 椎板切除术；c) 坐骨神经损伤；d) 假手术。每个样本采用20只小鼠的坐骨神经背根神经节混合样本。收集背根神经节后即刻进行极速冻存。