Magnetic control of GFP-like fluorescent proteins - Raw data 2

We've discovered a simple, nontoxic, biocompatible way to control the brightness of GFP-like fluorescent proteins via modest magnetic fields (~10 mT). Fluorescent proteins which seem magnetically inert (e.g. EGFP, mScarlet) become magnetoresponsive in the presence of an appropriate cofactor (e.g. EGFP-FlavinTag, or an mScarlet/FMN solution). This method works at room-temperature and body-temperature, in vitro, in E. coli and in cultured mammalian cells. The GFP-family magnetoresponse is weak (ΔF/F≈1%), but shows the hallmarks of evolvability. This suggests exciting technological possibilities, both short-term (e.g. lock-in detection, multiplexing) and long-term (e.g. optically-detected MRI, magnetogenetics). We've also discovered weak magnetoresponse from a member of the LOV-domain family. This suggests the possibility that magnetoresponse is a general feature of fluorescent proteins, and not unique to the cryptochrome/photolyase family. This repository holds some of the raw data for the main text figures. For the contents of the paper, please see: doi.org/10.5281/zenodo.8137174本研究发现了一种简便、无毒且生物相容的方法，可通过适度磁场（约10 mT）调控类绿色荧光蛋白（GFP-like fluorescent proteins）的发光亮度。原本看似无磁响应的荧光蛋白（如增强型绿色荧光蛋白（EGFP）、mScarlet）在添加合适辅因子（如EGFP-FlavinTag或mScarlet/黄素单核苷酸（FMN）混合体系）后，可获得磁响应特性。该方法可在室温、体温条件下，于体外（in vitro）、大肠杆菌（E. coli）以及培养的哺乳动物细胞中生效。 GFP家族蛋白的磁响应强度较弱（荧光强度相对变化量ΔF/F≈1%），但具备可进化性的典型特征。这为该技术带来了诸多极具前景的应用场景：短期可用于锁相检测（lock-in detection）、多路复用（multiplexing）等方向，长期则可应用于光学检测磁共振成像（optically-detected MRI）、磁遗传学（magnetogenetics）等领域。 本研究同时在光、氧、电压敏感结构域（LOV-domain）家族的一个蛋白成员中发现了微弱的磁响应现象。这表明磁响应或许是荧光蛋白的一类通用特性，而非仅局限于隐花色素/光裂合酶（cryptochrome/photolyase）家族。 本数据集仓库包含了正文图表所用的部分原始数据。如需查看论文完整内容，请访问：doi.org/10.5281/zenodo.8137174