HSV-1 DM-tRNA-Seq

MRC5 cells were mock-infected, infected with ICP4 null mutant (n12), or infected with wildtype HSV-1 (KOS) at an MOI of 10 PFU/cell for twelve hours and DM-tRNA-seq was performed. Illumina sequencing was performed using NextSeq550 to generate 75 PE reads, adaptors are Illumina TruSeq 6 base.

MRC5细胞经模拟感染、感染ICP4基因缺失突变株（n12），或以10 PFU/细胞的感染复数（MOI）感染野生型单纯疱疹病毒1型（KOS株），感染时长为12小时；随后开展DM-tRNA测序（DM-tRNA-seq）实验。实验采用NextSeq550平台进行Illumina测序，生成75碱基对双端测序读段，所用测序接头为Illumina TruSeq 6碱基接头。