Gene silencing mediated by dsRNA reduces grey leaf spot disease in maize

The dataset contains the analysis of grey leaf spot disease severity scores for two spray-induced gene silencing (SIGS) trials performed on B73 maize infected with Cercospora zeina conidia. Disease severity was measured on a scale of S1 - S8, where S1 denotes no symptoms and S8 indicates the most severe disease symptoms. A positive disease control contained only C. zeina inoculum. The remaining plants were inoculated with C. zeina conidia and treated with either GFP double-stranded RNA (dsRNA) as a negative treatment control, or the test construct 3Cz dsRNA. In addition, this dataset includes the mean C. zeina conidial germination percentages calculated for C. zeina germinating in vitro on culture media (assessed with light microscopy) and in planta on maize leaves (assessed with scanning electron microscopy).

本数据集涵盖两项针对感染玉蜀黍尾孢（Cercospora zeina）分生孢子的B73玉米开展的喷雾诱导基因沉默（spray-induced gene silencing, SIGS）试验的灰斑病病害严重度评分分析。病害严重度采用S1至S8的分级标准进行量化评估，其中S1代表无可见病征，S8代表病情最为严重的病状。阳性病害对照组仅接种玉蜀黍尾孢接种物；其余供试植株均接种玉蜀黍尾孢分生孢子，并分别以绿色荧光蛋白双链RNA（GFP double-stranded RNA, dsRNA）作为阴性处理对照，或采用测试载体3Cz双链RNA进行处理。此外，本数据集还包含玉蜀黍尾孢在体外培养基（经光学显微镜评估）及植物体内玉米叶片（经扫描电子显微镜评估）上的分生孢子萌发率均值数据。