Twist expression in HMLE and breast cancer T47D cells

Twist is a key EMT inducer, expression of Twist will induce EMT in HMLE and breast tumor T47D cells By expressing Twist in HMLE and T47D cells, which lack the expression of Twist, will identify the genes regulated by Twist Expressing Twist in HMLE and T47D cells, stable clones were selected and treated with BET inhibitor JQ1 and RNA were prepared for microarray analysis

Twist是关键的上皮间质转化（epithelial-mesenchymal transition, EMT）诱导因子，其表达可在HMLE细胞与乳腺肿瘤T47D细胞中诱导上皮间质转化。鉴于HMLE与T47D细胞自身不表达Twist，通过在这两种细胞中异位表达Twist，可鉴定出受Twist调控的靶基因。我们将筛选获得的稳定表达Twist的HMLE及T47D细胞克隆用BET抑制剂JQ1进行处理，随后提取RNA用于基因微阵列（microarray）分析。