Neuronal development in NEIL3-deficient mice (hippocampus)

brain tissue from all subregions (CA1, CA3, dentate gyrus) of the hippocampal formation was isolated at postnatal day 8 and at an adult age (3-6months old animals) in wildtype and NEIL3-deficient mice (n=4 at p8 and n=3 at adult age). NEILs are DNA glycosylases potentially involved in transcription regulation and hippocampal neuronal maturation. RNA was extracted and sequenced. Wildtype (C57Bl6) and mice deficient in NEIL3 (C57Bl6-background) were used. Tissue was isolated exclusively at baseline, i.e. the animal was taken directly from its homecage without any behavioral intervention

本研究分别于出生后第8天与成年阶段（3~6月龄动物），采集野生型及NEIL3缺陷型小鼠的海马结构（hippocampal formation）全部亚区——CA1、CA3及齿状回（dentate gyrus）的脑组织；其中出生后第8天组样本量为4只，成年组为3只。NEIL家族蛋白属于DNA糖苷酶（DNA glycosylases），可能参与转录调控及海马神经元成熟过程。实验对提取的RNA进行测序。所用野生型小鼠为C57BL/6品系，NEIL3缺陷型小鼠均为C57BL/6背景。组织仅在基线状态下取材，即直接从动物饲养笼中取样，未施加任何行为学干预。