Bulk RNA-seq of mouse colonic organoids: CRISPR functional Screening of Satb2-interact proteins (NuRD and SWI/SNF complex)

CRISPR knock out the NuRD and SWI/SNF complex gene from mouse colonic organoids to validate the potential function with SATB2. Overall design: Genomic modified mouse colonic organoids (Ctrl, Satb2, Mta2, Chd4, Gatad2a, Smarca5, Smarca4, Smarcd2 and Ctbp2 sgRNAs) were differentiated and collected for Bulk RNA-Seq

本研究通过CRISPR基因编辑技术，从小鼠结肠类器官中敲除NuRD复合物（NuRD complex）与SWI/SNF复合物（SWI/SNF complex）的编码基因，以验证其与SATB2的潜在功能关联。
总体实验设计：对经基因组修饰的小鼠结肠类器官（分别转导对照（Ctrl）、Satb2、Mta2、Chd4、Gatad2a、Smarca5、Smarca4、Smarcd2及Ctbp2单引导RNA（sgRNA））进行分化培养并收集样本，随后开展批量RNA测序（Bulk RNA-Seq）。