IFN-Gamma keratinocytes

An experiment comparing control versus IFN-g treated skin cells (one design factor on two levels). To evaluate the biological and the experimental variability, the following experimental design was planned as shown in Figure 1: "Control" and "IFN-g stimulated" keratinocytes were cultured in triplicate using three individual petri Æ10 culture dishes and RNA was independently extracted from each cultured replicate. For each RNA sample, cRNA synthesis was performed in triplicate and each cRNA pool was hybridized to an Affymetrix U133 Gene Chip. Keywords: ordered

本实验旨在对比对照组与干扰素γ（IFN-γ）处理的皮肤细胞，实验仅包含一个两水平的设计因子。为评估生物学变异与实验变异，我们按图1所示规划了如下实验方案：将"对照组"与"干扰素γ刺激组"的角质形成细胞（keratinocytes）使用3个独立的Æ10培养皿开展三次重复培养，并从每一份培养重复样本中独立提取RNA。针对每份RNA样本，我们进行三次重复的互补RNA（complementary RNA）合成，并将每一份cRNA混合样本与Affymetrix U133基因芯片进行杂交。关键词：有序