Supplementary material 3 from: Ceballos-Escalera A, Richards J, Arias MB, Inward DJG, Vogler AP (2022) Metabarcoding of insect-associated fungal communities: a comparison of internal transcribed spacer (ITS) and large-subunit (LSU) rRNA markers. MycoKeys 88: 1-33. https://doi.org/10.3897/mycokeys.88.77106

Figure S3. Maximum-likelihood tree constructed in IQ-Tree2 based on three-gene (LSU D1-D2, SSU, ITS2) reference sequence alignments and OTUs for both markers (clustering thresholds: 99% LSU D1-D2 and 98% ITS2)

补充图S3：采用IQ-Tree2软件，基于三个基因（大亚基D1-D2区域（LSU D1-D2）、小亚基（SSU）、内转录间隔区2（ITS2））的参考序列比对结果，以及对应两个标记基因（聚类阈值：大亚基D1-D2区域为99%、内转录间隔区2为98%）的操作分类单元（Operational Taxonomic Unit，OTU）构建的最大似然系统发育树