Human skeletal muscle transcriptome. Human skeletal muscle transcriptome

The annotation of the Affymetrix HTA 2.0 array was updated to optimise the detection of both coding and non-coding RNA in human skeletal muscle biopsy samples by removing invalid and low signal-high-variance probes. A transcript level CDF specific to skeletal muscle is provided to use within the standard aroma.affymetrix pipeline. Overall design: 527 human muscle profiles from men and women aged 18 to 95yrs. There are three age groupings: young adult (18-39), middle-aged adult (40-60), older adult (61+).

本数据集对Affymetrix HTA 2.0基因芯片（Affymetrix HTA 2.0 array）的注释信息进行了更新，通过移除无效探针与低信号高变异探针，以优化人类骨骼肌活检样本中编码RNA及非编码RNA的检测效能。本数据集提供了一款专为骨骼肌设计的转录本水平芯片定义文件（Chip Definition File, CDF），可在标准aroma.affymetrix分析流程中使用。整体实验设计：共纳入527份人类肌肉表达谱样本，供体年龄跨度为18至95岁，涵盖男性与女性。样本被划分为三个年龄组别：青年成人组（18-39岁）、中年成人组（40-60岁）以及老年成人组（61岁及以上）。