Identification of DDAH1 interacting proteins

To identify intracellular DDAH1-interacting proteins, a co-IP/MS assay was performed with MCF-10A cells stably expressing DDAH1-3×Flag.为鉴定细胞内与二甲基精氨酸二甲基氨基水解酶1（DDAH1）相互作用的蛋白质，本研究以稳定表达带3×Flag标签的DDAH1的MCF-10A细胞为材料，开展了免疫共沉淀-质谱联用（co-IP/MS）实验。