Schizochytrium mangrovei PQ6 transcriptome

The heterotrophic marine microalga Schizochytrium mangrovei PQ6 synthesizes large amounts of polyunsaturated fatty acids (PUFAs) with possible food and fuel applications. We characterized the transcriptome of S. mangrovei PQ6, focusing on lipid metabolism pathways throughout growth. Maximum lipid accumulation in 500 mL batch cultures occurred on day 5, with total lipid and DHA contents reaching 33.20±1.25% of dry cell weight (DCW) and 135.91 mg/g of DCW, respectively. We identified 30,782 unigenes from the transcriptomes of samples collected on day 1, 3, and 5, with an average size of 672.7 bp and N50 of 950 bp. A total of 9,980 unigenes were annotated in public protein databases. We identified 155 unigenes involved in lipid metabolism whose expression patterns corresponded with total lipid and DHA accumulation patterns, shedding light on the possible roles of PUFA pathways, such as those mediated by fatty acid synthase, polyketide synthase, and desaturase/elongase, in Schizochytrium.

异养海洋微藻红树林裂壶藻（Schizochytrium mangrovei）PQ6可大量合成多不饱和脂肪酸（polyunsaturated fatty acids, PUFAs），该类物质在食品与燃料领域具备潜在应用价值。本研究针对该菌株的转录组开展解析，重点聚焦其生长全过程中的脂质代谢通路。在500 mL分批培养体系中，脂质积累量于第5天达到峰值，总脂质与二十二碳六烯酸（DHA）含量分别可达细胞干重（dry cell weight, DCW）的33.20±1.25%，以及135.91 mg/g细胞干重。本研究从第1、3、5天采集的样本转录组中共鉴定得到30782个单基因簇（unigenes），其平均长度为672.7 bp，N50值为950 bp。共有9980个单基因簇在公共蛋白质数据库中获得功能注释。本研究鉴定出155个参与脂质代谢的单基因簇，其表达模式与总脂质及DHA的积累模式高度契合，为解析裂壶藻中多不饱和脂肪酸合成通路（如脂肪酸合酶、聚酮合酶以及去饱和酶/延长酶介导的通路）的潜在功能提供了理论依据。