Data underlying the research project: Genotype-specific reference interval of haptoglobin tests on the BN II System

This study was designed to investigate the reference intervals of serum HP on the BN II System for different genotype individuals. The data submitted are the basic conditions and clinical laboratory test data of 1229 healthy people, including Gender, Age, white blood cell (WBC), hemoglobin (HGB), alanine transaminase (ALT), glucose (GLU), cholesterol (CHO), Triglyceride (TG), low-density lipoprotein (LDL), haptoglobin concentration and genotype. Before blood collection, individuals were instructed to maintain a normal diet within 3 days and fast overnight (>8 hours). The exclusion criteria were set as follows: WBC>9.5 G/L, HGB<90 g/L, ALT>50 U/L, CHO≥5.69 mmol/L, TG≥2.26 mmol/L, LDL≥4.14 mmol/L, GLU>6.1 mmol/L. Serum HP concentrations were measured by BN II System (Siemens AG, Munich, Germany). WBC, HB levels were determined by XN-2000 (Sysmex, Kobe, Japan). ALT, CHO, TG, LDL, GLU measurements were determined by AU 5800 (Beckman Coulter, Brea, USA). HP genotyping was determined by gel electrophoresis method and real-time PCR method.

本研究旨在探究基于BN II系统检测的血清结合珠蛋白（haptoglobin，HP）参考区间在不同基因型个体中的分布情况。本次提交的数据涵盖1229名健康个体的基础信息与临床实验室检测指标，具体包括性别、年龄、白细胞（white blood cell，WBC）、血红蛋白（hemoglobin，HGB）、丙氨酸氨基转移酶（alanine transaminase，ALT）、葡萄糖（glucose，GLU）、胆固醇（cholesterol，CHO）、甘油三酯（triglyceride，TG）、低密度脂蛋白（low-density lipoprotein，LDL）、结合珠蛋白浓度及基因型。所有受试者在采血前3日内需维持正常饮食，且需隔夜禁食（时长＞8小时）。本研究设定的排除标准如下：白细胞＞9.5 G/L、血红蛋白＜90 g/L、丙氨酸氨基转移酶＞50 U/L、胆固醇≥5.69 mmol/L、甘油三酯≥2.26 mmol/L、低密度脂蛋白≥4.14 mmol/L、葡萄糖＞6.1 mmol/L。血清HP浓度采用BN II系统（Siemens AG，德国慕尼黑）进行检测；白细胞与血红蛋白水平通过XN-2000血液分析仪（Sysmex，日本神户）测定；丙氨酸氨基转移酶、胆固醇、甘油三酯、低密度脂蛋白及葡萄糖的检测均采用AU 5800全自动生化分析仪（Beckman Coulter，美国布雷亚）完成；HP基因型检测则采用凝胶电泳法与实时荧光定量PCR（real-time PCR）法。