Table_5_Transcriptomic and Metabolomic Analyses of Diaphorina citri Kuwayama Infected and Non-infected With Candidatus Liberibacter Asiaticus.XLSX

The Asian citrus psyllid Diaphorina citri is the transmission vector of Huanglongbing (HLB), a devastating disease of citrus plants. The bacterium “Candidatus Liberibacter asiaticus” (CLas) associated with HLB is transmitted between host plants by D. citri in a circulative manner. Understanding the interaction between CLas and its insect vector is key for protecting citrus cultivation from HLB damage. Here, we used RNA sequencing and liquid chromatography-mass spectrometry (LC-MS) to analyze the transcriptome and metabolome of D. citri interacting with CLas. We identified 662 upregulated and 532 downregulated genes in CLas-infected insects. These genes were enriched in pathways involving carbohydrate metabolism, the insects’ immune system, and metabolism of cofactors and vitamins. We also detected 105 differential metabolites between CLas-infected and non-infected insects, including multiple nucleosides and lipid-related molecules. The integrated analysis revealed nine pathways—including those of the glycine, serine, threonine, and purine metabolism—affected by the differentially expressed genes from both groups. The network for these pathways was subsequently constructed. Our results thus provide insights regarding the cross-talk between the transcriptomic and metabolomic changes in D. citri in response to CLas infection, as well as information on the pathways and genes/metabolites related to the CLas–D. citri interaction.

亚洲柑橘木虱（Diaphorina citri）是柑橘毁灭性病害黄龙病（Huanglongbing, HLB）的传播媒介。与HLB相关的“亚洲韧皮部杆菌属候选种”（Candidatus Liberibacter asiaticus, CLas）可通过D. citri以循回式传播方式在寄主植物间传播。解析CLas与其昆虫媒介之间的互作机制，是保护柑橘栽培免受HLB危害的关键所在。本研究采用RNA测序（RNA sequencing）与液相色谱-质谱联用法（liquid chromatography-mass spectrometry, LC-MS），对CLas侵染状态下D. citri的转录组（transcriptome）与代谢组（metabolome）开展分析。研究共在CLas侵染的木虱体内鉴定出662个上调基因与532个下调基因，上述差异表达基因（differentially expressed genes）富集于碳水化合物代谢、昆虫免疫系统以及辅因子与维生素代谢等通路。此外，本研究还在CLas侵染与未侵染的木虱样本中检测到105种差异代谢物（differential metabolites），涵盖多种核苷与脂类相关分子。整合分析（integrated analysis）结果显示，甘氨酸、丝氨酸、苏氨酸代谢及嘌呤代谢等9条通路，均受到两组样本间差异表达基因的调控，研究团队随后构建了这些通路的互作网络。本研究结果不仅为解析D. citri响应CLas侵染时转录组与代谢组变化间的交叉互作（cross-talk）提供了全新视角，同时也为CLas与D. citri互作相关的通路、基因及代谢物研究提供了详实的参考数据。