ego3-Studying the transcriptional consequences of a QTL for shoot growth (SG3, SG1). Studying the transcriptional consequences of a QTL for shoot growth under mannitol condition (EGO3).. Arabidopsis thaliana

ra09-01_qtlleafgrowth - ego3 - What are the transcriptional consequences of the allelic contrast at SG3, SG1 and EGO3 loci mapped for its effect on shoot growth and identified at the gene level - Plants were grown in the in vitro conditions with 60 mM mannitol, where we have mapped and cloned the concerned QTL which allows us to see the phenotype linked to the segregation of the natural alleles. We are comparing 2 genotypes descending from a RIL from the Cvi x Col (H1 is Col allele containing HIF, and A2 is Cvi allele containing HIF). Overall design: 2 dye-swap - genotype comparison

数据集标识：ra09-01_qtlleafgrowth - ego3。本研究旨在探究定位在调控茎生长且已在基因层面鉴定的SG3、SG1与EGO3基因座上的等位基因差异所产生的转录组效应。实验植株均培养于添加60 mM甘露醇的体外培养体系中，本研究在此体系中完成了目标数量性状基因座（Quantitative Trait Locus, QTL）的定位与克隆，借此可观测与自然等位基因分离相关的表型。本研究比较的两个基因型均源自Cvi生态型与Col生态型杂交获得的重组近交系（Recombinant Inbred Line, RIL）：其中H1为携带HIF的Col等位基因，A2为携带HIF的Cvi等位基因。实验整体设计：采用2次染料互换的基因型比较实验方案。