Expression profiling by high-throughput sequencing to evaluate effects of aroylated phenylenediamine HO53 on transcriptome in human undifferentiated bronchial epithelial cells (BCi-NS1.1 cell line).

We used RNA sequencing to exploit molecular mechanisms behind HO53-induced innate immune responses in undifferentiated human bronchial epithelial cells (BCi-NS1.1 cell line). We performed transcriptome analysis of undifferentiated bronchial epithelial cells (BCi-NS1.1 cell line) treated with aroylated phenylenediamine HO53 for different time points 4 h, 8 h and 24 h and compared it to transcriptome of untreated cells. The broad effect of HO53 on the gene expression in undifferentiated human lung epithelial cells (BCi-NS1.1) points towards an epigenetic event. Furthermore, genes involved in metabolic pathways (glycolysis, TCA cycles, pentose phosphate, glycosylation and gluconeogenesis) were particularly affected by HO53 treatement. The expression pattern for few relevant selected genes was reconfirmed by qRT-PCR using SYBR Green reagent. Overall design: Expression profiling by high-throughput sequencing of HO53-treated vs untreated undifferentiated human bronchial epithelial cells (BCi-NS1.1 cell line).

本研究通过RNA测序（RNA sequencing），探究了未分化人支气管上皮细胞（BCi-NS1.1细胞系）中HO53诱导的先天免疫应答背后的分子机制。我们对经芳酰基苯二胺HO53处理4小时、8小时及24小时的未分化支气管上皮细胞（BCi-NS1.1细胞系）进行转录组分析，并与未处理细胞的转录组进行对比。HO53对未分化人肺上皮细胞（BCi-NS1.1）基因表达的广泛影响，提示其作用机制可能涉及表观遗传调控事件。此外，参与糖酵解、三羧酸循环、磷酸戊糖途径、糖基化及糖异生等代谢通路的基因，均受HO53处理的显著影响。我们采用SYBR Green试剂通过qRT-PCR，对筛选出的部分相关基因的表达模式进行了验证。实验整体设计：通过高通量测序，对经HO53处理与未处理的未分化人支气管上皮细胞（BCi-NS1.1细胞系）开展表达谱分析。