Biologic characteristics of B-lymphoblastic leukemia correspond to immunoglobulin gene diversity [array]

We tested the relationship between IgH subclone diversity in B-ALL and the number of resolved chromosomal breaks as assessed by chromosome microarray analysis (CMA). Given the role of off-target recombination activating gene (RAG) activity at non-IgH sites in promoting copy number aberrations (CNA) in B-ALL, we conducted CMA to test whether patients with RAG-mediated IgH subclone diversity demonstrate greater karyotypic variability.

我们探究了B细胞急性淋巴细胞白血病（B-cell Acute Lymphoblastic Leukemia, B-ALL）中免疫球蛋白重链（Immunoglobulin Heavy Chain, IgH）亚克隆多样性与通过染色体微阵列分析（Chromosome Microarray Analysis, CMA）评估的经解析染色体断裂数量之间的关联。鉴于非IgH位点的脱靶重组激活基因（Recombination Activating Gene, RAG）活性在促进B-ALL拷贝数异常（Copy Number Aberrations, CNA）中的作用，我们通过CMA实验验证了携带RAG介导的IgH亚克隆多样性的患者是否呈现出更显著的核型变异性。