Application of COF nanomaterial carrier in root disease control and its role in plants

With the rapid development of nanotechnology, it has been widely penetrated into various fields, and the application of nanocarriers in drug delivery systems is becoming more and more mature. In this study, soil rhizosphere disease (root rot) was taken as the research object, and chemical pesticides were delivered by nanocarriers to achieve efficient control effect. Through the combination of COF carrier and fluomycetin, a highly effective fungicide against pathogenic bacteria of root rot disease (COF@FLU) was successfully constructed. The particle diameter of COF@FLIU was *, the encapsulation rate of active ingredients was *, and the antibacterial rate was 45.3% higher than that of free FLU, but COF itself had no antibacterial activity. Compared with the control group, the plant height, leaf area, root length, antioxidant enzymes and reactive oxygen species were increased by 34%, 54%, 27%, 66% and 52% respectively. The transcriptome found that COF vector induced gene expression changes in plants. These genes are mainly enriched in MAPK signaling pathway involved in cell proliferation and differentiation, Carbon fixation in photosynthetic organisms pathway that maintains carbon cycle and improves photosynthesis, and Plant that regulates growth and development hormone signal transduction pathway, etc. According to the metabolome, the differential abundance of metabolites in plants induced by COF was mainly enriched in Cysteine and methionine metabolism, which is involved in protein synthesis and antioxidant defense, and Lipoic acid metabolism, which is related to energy metabolism. And enzyme catalyzed Biosynthesis of cofactors. Combined transcriptome and metabolome analysis further revealed that COF can regulate photomorphogenesis and environmental adaptation, thereby promoting plant growth. This study proved the high efficiency of COF nanocarriers in soil rhizosphere disease control, and also provided a scientific basis for the application of COF in plant growth regulation.

随着纳米技术的飞速发展，其已广泛渗透至诸多领域，纳米载体在药物递送系统中的应用也日趋成熟。本研究以土壤根际病害（根腐病）为研究对象，采用纳米载体递送化学农药以实现高效防控效果。通过将COF（Covalent Organic Framework）载体与氟霉素（fluomycetin）结合，成功构建了针对根腐病病原菌的高效杀菌剂（COF@FLU）。COF@FLU的粒径为*，活性成分包封率为*，抑菌率较游离氟霉素（FLU）提升45.3%，但COF本身不具备抑菌活性。与对照组相比，植株株高、叶面积、根长、抗氧化酶活性及活性氧含量分别提升34%、54%、27%、66%和52%。转录组分析显示，COF载体可诱导植株体内基因表达发生改变。这些差异表达基因主要富集于参与细胞增殖与分化的MAPK信号通路、维持碳循环并提升光合效率的光合生物碳固定通路，以及调控植物生长发育的激素信号转导通路等。代谢组分析表明，COF诱导的植株差异代谢物主要富集于参与蛋白质合成与抗氧化防御的半胱氨酸与甲硫氨酸代谢、与能量代谢相关的硫辛酸代谢，以及酶催化的辅因子生物合成通路。转录组与代谢组联合分析进一步揭示，COF可通过调控植物光形态建成与环境适应性，进而促进植株生长。本研究证实了COF纳米载体在土壤根际病害防控中的高效性，同时为COF在植物生长调控领域的应用提供了科学依据。