Transcriptome sequencing of wild-type cucumber 10Y and mutant npf1, and genome-wide identification of CsNPF1 targets in Cucumber

A transcriptome deep sequencing (RNA-seq) analysis was performed using ovaries collected from 10Y and npf1 plants in order to reveal the molecular mechanisms that underlie CsNPF1-controlled parthenocarpy. Additionally, a Dap-Seq study was conducted to identify the genome-wide binding patterns of CsNPF1 and to investigate its role in regulating parthenocarpy in cucumber.

本研究以10Y与npf1植株的子房为材料，开展转录组深度测序（RNA-seq）分析，以揭示CsNPF1调控黄瓜单性结实的分子机制。此外，本研究还通过DNA亲和纯化测序（DAP-seq）实验，鉴定CsNPF1的全基因组结合模式，并解析其在黄瓜单性结实调控中的作用。