The Hippo pathway effector YAP is an essential regulator of submandibular gland ductal progenitor patterning. Mus musculus

To investigate the roles of YAP in the submandibular gland (SMG) epithelium, we compared the gene expression profiles of E15.5 SMGs isolated from Yap-loxP/loxP or Yap-loxP/loxP;Shh-Cre (i.e. Cre recombinase from the Shh promoter) embryos. Overall design: We collected RNA from E15.5 SMGs isolated from Yap-loxP/loxP and Yap-loxP/loxP;Shh-Cre litter mate mice. Embryonic SMGs from three separate timed matings were used for this experiment.

为探究YAP在颌下腺（submandibular gland, SMG）上皮中的作用，我们对比了分别从Yap-loxP/loxP或Yap-loxP/loxP;Shh-Cre（即由Shh启动子驱动表达的Cre重组酶）胚胎中分离的E15.5龄颌下腺的基因表达谱。实验总体设计：我们从Yap-loxP/loxP与Yap-loxP/loxP;Shh-Cre同窝小鼠中分离获得E15.5龄颌下腺并提取RNA。本实验采用了三次独立定时交配得到的胚胎颌下腺样本。