The splicing activator DAZAP1 integrates splicing control into MEK/Erk regulated cell proliferation and migration. Homo sapiens

DAZAP1 was depleted in culterd HEK 293T cells using shRNA and the resulting poly A RNA were isolated c-DNA library constructed and paired end sequenced on illumina Hi-seq 2000 platform the data was compared to a control shRNA depleted cell Overall design: Gene expression and splicing switches upon DAZAP1 knockdown

本研究采用短发夹RNA（shRNA）在培养的HEK 293T细胞中敲低DAZAP1基因，随后分离得到聚腺苷酸RNA（poly A RNA），构建互补DNA（cDNA）文库，并在Illumina HiSeq 2000测序平台上完成双端测序。将所得测序数据与转染对照shRNA的细胞对照组进行比对分析。整体实验设计：DAZAP1基因敲低后的基因表达与可变剪接切换事件。