Infected hepatocytes present a PbCSP-specific epitope to primed CD8+ T-cells and protect mice against SPZ challenge.

Each recipient TAP−/− mouse (H-2Kb) received 7×105PbSPZ-infected BALB/c (H-2kd) hepatocytes by IS transfer as described in Materials and Methods. Before IS injection, infected hepatocytes were isolated from BALB/c mice that were injected (iv) with 106 ANKA wt PbSPZ 2 h earlier. C7 and S14 (20 million cells per mouse, iv) were injected into the corresponding group 4 h after IS transfer. Mice were protected if they remained parasite negative 2 weeks after infected hepatocyte transfer.

每只TAP−/−小鼠（TAP−/− mouse，H-2Kb基因型）均按照《材料与方法》所述的实验流程，通过脾内转移（IS transfer）输注7×10^5个感染伯氏疟原虫子孢子（PbSPZ）的BALB/c（H-2kd基因型）肝细胞。在进行脾内注射前，感染肝细胞需从2小时前经静脉注射（intravenous, iv）10^6个ANKA野生型PbSPZ的BALB/c小鼠体内分离得到。脾内转移后4小时，分别向对应组别小鼠经静脉注射C7与S14制剂，每只小鼠输注2×10^7个细胞。若受感染肝细胞转移后2周小鼠仍呈寄生虫阴性，则判定小鼠获得免疫保护。