Alcohol-specific transcriptional dynamics of memory reconsolidation

To assess gene expression underlying alcohol memory in different regions of the brain, we performed total RNA-sequencing (RNA-seq) on brain tissue (prefrontal cortex or dorsal hippocampus) from C57BL/6 adult mice that had undergone alcohol placement conditioning and either placed back into alcohol context (retrieval, Ret) or briefly handled as a control (no retrieval, NoRet) 2-3 biological replicates, each consisting of prefrontal cortex or dorsal hippocampi from individual mice. Pooled stranded libraries were run on Illumina Hiseq 2000, single-end, 50 bp reads. Data was processed by Bowtie2, Tophat2, featurecounts, and DESeq2 to assess differential gene expression.

为探究不同脑区中酒精记忆相关的基因表达调控规律，我们对经酒精位置条件化训练的C57BL/6成年小鼠的脑组织（前额叶皮层或背侧海马，prefrontal cortex、dorsal hippocampus）开展总RNA测序（total RNA-sequencing, RNA-seq）。实验小鼠分为两组：一组被放回酒精环境以触发记忆提取（retrieval, Ret），另一组仅接受简易操作作为对照（无记忆提取，NoRet）；每组设置2-3个生物学重复，每个重复均取自单只小鼠的前额叶皮层或背侧海马组织。我们采用链特异性混合文库，在Illumina Hiseq 2000平台上进行单端测序，测序读长为50 bp。测序数据通过Bowtie2、Tophat2、featureCounts及DESeq2完成处理，以分析差异基因表达情况。