Single cell RNA sequencing of treatment naive Crohn's disease

Crohn's disease (CD) is characterized by chronic inflammation throughout the intestine. Previous single cell transcriptomic studies investigated the effect of CD post therapeutic intervention, however, the extent to which therapy modifies the intestinal inflammatory environment remains to be elucidated. To this end, a treatment naive pediatric CD cohort was recruited to investigate initial disease onset across the intestines, highlighting shared and tissue-specific consequences of disease. A clustering stability assessment workflow was developed to ensure clustering and downstream results were robust. Tensor decomposition was utilized to identify clinically meaningful groups within the intestines.

克罗恩病（Crohn's Disease，CD）以全肠道慢性炎症为主要特征。既往单细胞转录组学研究已探讨了克罗恩病患者接受治疗干预后的相关影响，但治疗手段在多大程度上可改变肠道炎症微环境，仍有待进一步阐明。为此，本研究招募了未接受过治疗的儿童克罗恩病队列，以探究疾病初始发作状态下全肠道的分子特征，明确疾病共有的及组织特异性的病理后果。本研究开发了一套聚类稳定性评估流程，以确保聚类分析及后续下游分析结果具备良好的鲁棒性。本研究采用张量分解技术，识别肠道组织中具有临床意义的患者亚群。