The influence of Aloe vera with mesenchymal stem cells from dental pulp on bone regeneration: characterization and treatment of non-critical defects of the tibia in rats

Abstract Objective This study aimed to evaluate the inflammatory effect and bone formation in sterile surgical failures after implantation of a collagen sponge with mesenchymal stem cells from human dental pulp (hDPSCs) and Aloe vera. Material and Methods Rattus norvegicus (n=75) were divided into five experimental groups according to treatment: G1) control (blood clot); G2) Hemospon®; G3) Hemospon® in a culture medium enriched with 8% Aloe vera; G4) Hemospon® in a culture medium containing hDPSCs and G5) Hemospon® in a culture medium enriched with 8% Aloe vera and hDPSCs. On days 7, 15 and 30, the animals were euthanized, and the tibia was dissected for histological, immunohistochemistry and immunofluorescence analyses. The results were analyzed using nonparametric Kruskal-Wallis test and Dunn’s post-test. Results On days 7 and 15, the groups with Aloe vera had less average acute inflammatory infiltrate compared to the control group and the group with Hemospon® (p

【摘要】 研究目的：本研究旨在评估植入含人牙髓间充质干细胞（human dental pulp stem cells, hDPSCs）与芦荟的胶原海绵后，无菌性手术失败相关的炎症反应与骨形成情况。材料与方法：将75只褐家鼠（Rattus norvegicus）按处理方式分为5个实验组：G1为对照组（血凝块组）；G2为Hemospon®组；G3为采用添加8%芦荟的培养基配制的Hemospon®组；G4为采用含hDPSCs的培养基配制的Hemospon®组；G5为采用添加8%芦荟且含hDPSCs的培养基配制的Hemospon®组。分别于术后第7、15、30天处死动物，剥离胫骨以开展组织学、免疫组织化学及免疫荧光分析。研究结果采用非参数克鲁斯卡尔-沃利斯（Kruskal-Wallis）检验与邓恩（Dunn）事后检验进行统计学分析。结果：在第7天与第15天，含芦荟的实验组的急性炎症浸润平均程度低于对照组与Hemospon®组（p