RNA sequencing from inflorescence meristems of three Arabidopsis thaliana mutant lines: puchi x bop1x bop2 ; lfy-12 and jagged-5D

RNA sequencing from micro-dissected meristems of the main shoot of the following mutant lines: the triple mutant puchi-1 x bop1-4 x bop2-11 (PB); the single mutant lfy-12 (L-12), and the single mutant jagged-5D (J5D) at early floral stage (floral transition occured but meristems were sampled before the plants had bolted). These three mutants are in a Col-0 genetic background. Three independant replicates (1, 2, 3) per genotype are provided, each replicate is a pool of ~10 apices. Plants were cultured in classical Arabidopsis growth conditions in culture chambers, starting with 21 days in short days before switching to long-day conditions untill dissection and sampling (See Dieudonné S. et al. for details). cDNA was sequenced using NextSeq500 from Illumina. After classical read quality filtering, each sample yielded on average 43 million sequences.

本数据集针对以下突变体株系的主茎微解剖分生组织开展RNA测序（RNA sequencing）：三重突变体puchi-1 × bop1-4 × bop2-11（标记为PB）、单突变体lfy-12（标记为L-12）以及单突变体jagged-5D（标记为J5D），采样时点为早花阶段——即植株已发生花转变但尚未抽薹时采集其分生组织。上述三个突变体均处于Col-0遗传背景中。每个基因型设置3次独立生物学重复（编号1、2、3），每个重复样本为约10个茎尖的混合样品。植株在标准拟南芥培养条件下于培养箱中培养：先在短日照条件下培养21天，随后切换至长日照条件，直至完成解剖与采样（详细实验流程参见Dieudonné S.等的研究文献）。使用Illumina公司NextSeq500平台对cDNA进行测序。经过标准读段质量过滤后，每个样本平均产出4300万条序列。