The real-time RT-PCR results of HEV71, CVA16, and HEVs for different types of HFMD clinical specimens.

Note:
1. *Among 48 HEVs-positive specimens, one specimen was positive for HEVs, but negative for HEV71 or CVA16, which was identified as Echo11 by virus isolation, RT-PCR, and sequencing for the VP1 gene.
2. For the positive specimens for HEV71 or CVA16, the results of real-time RT-PCR for HEVs were also positive.
3. A total of 85 HEV71 and 16 CVA16 were identified using RT-PCR method among the 213 HFMD clinical specimens, which were lower than those of the real-time RT-PCR assay.
4. The multiplex real-time RT-PCR assay was shown to exhibit 100% specificity in detecting HEV71 and CVA16, which was confirmed by sequencing.

备注：
1. 在48份人类肠道病毒（Human Enteroviruses, HEVs）阳性标本中，有1份标本HEVs检测呈阳性，但肠道病毒71型（HEV71）与柯萨奇病毒A16型（CVA16）检测均呈阴性，经病毒分离、VP1基因逆转录聚合酶链反应（Reverse Transcription-Polymerase Chain Reaction, RT-PCR）及测序鉴定为埃可11型（Echo11）。
2. 对于肠道病毒71型（HEV71）或柯萨奇病毒A16型（CVA16）呈阳性的标本，其人类肠道病毒实时逆转录聚合酶链反应（real-time RT-PCR）检测结果亦为阳性。
3. 在213份手足口病（Hand, Foot and Mouth Disease, HFMD）临床标本中，经RT-PCR方法共鉴定出85株HEV71与16株CVA16，该检出量低于实时RT-PCR检测方法的结果。
4. 经测序验证，该多重实时RT-PCR检测方法在检测HEV71与CVA16时表现出100%的特异性。