Data on soil physicochemical and microbiological properties in topsoil and subsoil of a Mediterranean irrigated orchard

Data on an experiment to find out the interconnections between soil physicochemical properties and the microbial communities residing in the topsoil and the subsoil of irrigated orchards under a semiarid Mediterranean climate. We collected soil samples from two depths, topsoil (0-10 cm) and subsoil (30-50 cm), noting elevated salinity levels in the topsoil due to irrigation practices. Utilizing high-throughput 16S rRNA gene and ITS1 region amplicon sequencing, we characterized the bacterial and fungal communities across these two depths.本数据集对应一项实验的相关数据，旨在揭示半干旱地中海气候条件下灌溉果园表层土壤（topsoil）与深层土壤（subsoil）的理化性质，与其内定殖的微生物群落之间的内在关联。研究团队采集了两个深度层级的土壤样品：表层土壤（0-10 cm）与深层土壤（30-50 cm），并观测到灌溉活动导致表层土壤盐度升高的现象。本研究采用高通量16S核糖体RNA基因（high-throughput 16S rRNA gene）与ITS1区（ITS1 region）扩增子测序技术，对这两个深度层级土壤中的细菌与真菌群落进行了表征分析。