Chronic nicotine differentially affects murine transcriptome profiling in isolated cortical interneurons and pyramidal neurons. Mus musculus

Nicotine differentially affected subset of genes in Sst- and Thy1- neurons, which might contribute to the distinct effect of nicotine on interneuron and pyramidal neuron activities. Meanwhile, the altered transcripts associated with mitochondrial activity were found between interneurons and pyramidal neurons after chronic nicotine. Overall design: We assessed the differentially expressed genes (DEGs) by nicotine in Sst- or Thy1- neurons, respectively, and then compared DEGs between Sst- and Thy1- neurons in the absence and presence of nicotine.

尼古丁可对Sst-神经元与Thy1-神经元的部分基因产生差异化调控作用，这或可解释尼古丁对中间神经元与锥体神经元活性的差异化影响。同时，经慢性尼古丁处理后，中间神经元与锥体神经元之间出现了与线粒体活性相关的转录本表达改变。
实验整体设计：我们分别检测了尼古丁在Sst-神经元或Thy1-神经元中诱导的差异表达基因（differentially expressed genes, DEGs），随后对比了有无尼古丁处理条件下，Sst-神经元与Thy1-神经元之间的差异表达基因。