Escape from X-Inactivation Tumor Suppressor (EXITS) genes contribute to sex bias

In this manuscript, we described male-biased mutations in chrX genes in cancer. In this RNA-seq experiment we tested the transcriptional consequences of shRNA knockdown of one of those genes, CNKSR2 Murine NIH 3T3 cells were infected with and selected for expression of lentiviruses expressing shRNAs targeting Cnksr2 (2 independent shRNA sequences) or a control shRNA (targeting RFP, a gene not present in these cells). Each was performed in biological triplicate independent cultures for n=9 total samples

本研究手稿针对癌症中X染色体（chrX）基因的男性偏向性突变展开了阐述。在本次RNA测序（RNA-seq）实验中，我们针对上述基因之一CNKSR2开展了短发夹RNA（shRNA）介导的基因敲降实验，以探究其转录层面的生物学效应。将携带靶向Cnksr2的短发夹RNA（共2条独立序列）或靶向红色荧光蛋白基因（RFP，该细胞系中不存在该基因）的对照短发夹RNA的慢病毒（lentivirus）感染小鼠NIH 3T3细胞，并筛选出成功表达相应shRNA的阳性细胞。每组实验均设置3次独立生物学重复培养，最终总样本量为9例。