Epigenetic and genetic dissections of UV-induced global gene dysregulation in skin cells through multi-omics analyses

In this study, we performed integrative multi-omics studies to understand the complex mechanisms underlying UV photobiological effects. While H3K27ac and genetic mutations can both contribute to UV-induced transcriptomic changes, additional omics-based studies on other histone modifications, DNA methylation, and whole genome mapping of genetic mutations will provide a thorough understanding of UV-gene interactions in skin disease pathogenesis. The new UV target genes that we identified have important clinical implications in skin carcinogenesis.. In this study, we performed RNA-Seq, Chip-Seq and Exome-seq studies to elucidate the molecular mechanisms underlying the photobiological effects of UV radiation on gene regulation in skin homeostasis

本研究通过整合多组学（multi-omics）分析，旨在解析紫外线（UV）光生物学效应背后的复杂分子机制。尽管组蛋白H3第27位赖氨酸乙酰化（H3K27ac）与遗传突变均可介导紫外线诱导的转录组改变，但针对其他组蛋白修饰、DNA甲基化以及遗传突变全基因组图谱分析的补充组学研究，将有助于全面阐明皮肤疾病发病机制中紫外线与基因的相互作用。本研究鉴定得到的新型紫外线靶基因，在皮肤癌变进程中具有重要的临床意义。本研究通过RNA测序（RNA-Seq）、染色质免疫共沉淀测序（Chip-Seq）与外显子组测序（Exome-seq）实验，阐明了紫外线辐射对皮肤稳态中基因调控的光生物学效应背后的分子机制。