Single-cell profiling of histone modifications and transcription in developmental systems with EpiDamID [mouse ESC]. Single-cell profiling of histone modifications and transcription in developmental systems with EpiDamID [mouse ESC]

We developed EpiDamID, an adaptation of existing DamID protocols that extends their applicability to histone PTMs. Overall design: Profiling H3K27me3 and H4K20me1 in single mouse embryonic stem cells with scDam&T-seq.

本研究开发了表观基因组DamID技术（EpiDamID），该技术系现有DamID实验方案的改良版本，可将其应用范畴拓展至组蛋白翻译后修饰（histone PTMs）。实验设计概述：依托单细胞Dam&T测序（scDam&T-seq）技术，完成单个小鼠胚胎干细胞内H3K27me3与H4K20me1的谱学表征。