HMS-ESTEEM Analysis Dataset File, SAS in Human Microbiome Study-ESTEEM

HMS-ESTEEM Analysis Dataset File, SAS
Study Description
The overarching aim of the HMS-ESTEEM study was to describe the role that the urinary microbiome plays in disorders of the lower genitourinary system, namely, the female genitourinary microbiome. The primary aim of the study evaluated whether the urinary microbiome differs between women with mixed urinary incontinence (MUI) and unaffected controls. There were 212 women (128 affected and 84 controls) who participated in the study and had catheterized urine specimens, vaginal specimens sent for culture, and DNA analysis. From the catheterized urine samples obtained, v4–6 regions of the 16S rRNA gene were sequenced to identify bacteria. Women with MUI and controls did not differ in overall Lactobacillus predominance. In younger women, urinary bacterial community-types differentiated MUI from controls. This multi-site observational study included both a subset of patients enrolled in ESTEEM and unaffected controls (Total n = 212; MUI patients n = 128, Controls n = 84).

HMS-ESTEEM 分析数据集文件（SAS）
研究概况
HMS-ESTEEM研究的总体目标为阐明泌尿微生物组在下泌尿生殖系统疾病中的作用，具体聚焦于女性泌尿生殖微生物组。本研究的首要目标为评估混合性尿失禁（Mixed Urinary Incontinence, MUI）女性与健康对照者的泌尿微生物组是否存在差异。
本研究共纳入212名女性受试者，其中128例为混合性尿失禁患者，84例为健康对照者；所有受试者均留取导尿尿液标本、送检培养的阴道标本，并接受DNA分析。针对获取的导尿尿液标本，研究人员对16S rRNA基因（16S ribosomal RNA gene）的v4–6可变区进行测序，以鉴定样本中的细菌。
混合性尿失禁患者与健康对照者的乳杆菌（Lactobacillus）主导型菌群总体占比无显著差异。在年轻女性亚组中，尿液细菌群落型可有效区分混合性尿失禁患者与健康对照者。
此项多中心观察性研究的受试者涵盖纳入ESTEEM研究的部分患者与健康对照者，总样本量为212例，其中混合性尿失禁患者128例，健康对照者84例。