Additional file 1 of Small-scale sequencing enables quality assessment of Ribo-Seq data: an example from Arabidopsis cell culture

Additional file 1: Table S1. Biotinylated rRNA depletion probes. Table S2. List of rRNA databases used with SortMeRNA for rRNA removal. Table S3. Arabidopsis rRNA fasta file added to the SortMeRNA database. Table S4. Mapping quality comparison between bowtie 2 and kallisto. Table S5. Library amplification primers. Figure S1. Gel images of the library construction procedure. Figure S2. The rRNA compositions of the libraries. Figure S3. Genome browser (IGB) view of a randomly selected region. Figure S4. Ribo-Seq QC analysis of T0-2 and T0-3 data. Figure S5. Meta-gene heatmap of T0-2 and T0-3 data. Figure S6. The RPF profiles of the two most abundant transcripts.

附加文件1：表S1。生物素化核糖体RNA（rRNA）去除探针。表S2：配合SortMeRNA进行rRNA移除所用的核糖体RNA数据库列表。表S3：添加至SortMeRNA数据库的拟南芥核糖体RNA FASTA文件。表S4：Bowtie 2与kallisto的比对质量对比。表S5：文库扩增引物。图S1：文库构建流程的凝胶电泳图像。图S2：文库的核糖体RNA组成。图S3：随机选取区域的基因组浏览器（IGB）视图。图S4：T0-2与T0-3数据的核糖体测序（Ribo-Seq）质量控制分析。图S5：T0-2与T0-3数据的元基因热图。图S6：两个丰度最高转录本的核糖体保护片段（RPF）图谱。