Pelagic bacterial communities across Fram Strait

Water samples were collected in Fram Strait during the Polarstern cruise PS85 (June 6th 2014 until July 3rd 2014) from eastern Greenland shelf to west coast of Spitsbergen. For assessing bacterial community composition 2 L of water were filtered with peristaltic pump through successive membrane filters of 3 μm and 0.22 μm. The extracted genomic bacterial DNA was isolated and the hypervariable V3-V4 (341F - 785R) region of the bacterial 16S rRNA was amplified and sequenced using Illumina MiSeq platform in a 2 × 300 bp paired-end run.

本数据集的水样采集于弗拉姆海峡（Fram Strait）的极星号（Polarstern）科考航次PS85期间（2014年6月6日至2014年7月3日），采样范围覆盖格陵兰岛东部陆架至斯匹次卑尔根岛（Spitsbergen）西海岸。为解析细菌群落组成，使用蠕动泵（peristaltic pump）将2升水样依次通过孔径3 μm和0.22 μm的膜滤器（membrane filter）进行过滤。所提取的细菌基因组DNA经分离纯化后，以引物341F-785R扩增细菌16S核糖体RNA（16S rRNA）的高变V3-V4区，并采用Illumina MiSeq测序平台完成2×300 bp双端测序。