Transcriptome analysis of gene expression changes in fer-8 and the parental line

To reveal transcriptional changes in the fer-8 mutant that could explain the increase in Pseudomonas colonization, we performed transcriptional profiling in both shoots and roots from fer-8 and the parental line. We identified 675 up-regulated genes in the shoots of fer-8 relative to wildtype plants. Surprisingly, we found only 82 up-regulated genes in fer-8 roots relative to the parental line, and there were no significantly enriched GO terms. In contrast, we found that the genes upregulated in shoots were enriched in GO terms related to defense, response to fungi, and JA signaling, consistent with previous reports of JA activation in the shoots of another fer mutant fer-4. Using RNAseq approach to disect trangscriptome changes in roots and shoots in a novel mutant deficient in feronia receptor like kinase (fer-8).

为揭示可解释假单胞菌（Pseudomonas）定殖增加的fer-8突变体转录变化，我们对fer-8及其亲本株系的地上部与根部开展了转录谱分析。相较于野生型植株，我们在fer-8地上部中鉴定出675个上调基因。令人意外的是，相较于亲本株系，fer-8根部仅鉴定出82个上调基因，且未发现显著富集的基因本体（Gene Ontology，GO）术语。与之相反，地上部的上调基因富集于与防御反应、真菌响应以及茉莉酸（Jasmonic Acid，JA）信号通路相关的GO术语，这与此前关于另一FERONIA类受体激酶（FERONIA receptor-like kinase）突变体fer-4地上部茉莉酸激活的研究报道相符。本研究采用RNA测序（RNA Sequencing，RNA-seq）技术，解析了新型FERONIA类受体激酶缺陷突变体fer-8的根部与地上部转录组变化。