Next Generation Sequencing Facilitates Quantitative Analysis of LPS treated THP-1 cells' Transcriptomes

In order to understand the consequences of the LPS from different bacterias, we analyzed RNA gene expression profiles in a human myeloid cell line THP-1 cells. RNA-seq transcriptome was analyzed in THP-1 cells treated with LPS from three bacteria B. fungorum, S. marcescens, and P. myrsinacearum. Overall design: mRNA profiles of bacteria B. fungorum, S. marcescens, and P. myrsinacearum LPS treated THP-1 cells

为探究不同来源脂多糖（Lipopolysaccharide, LPS）的生物学效应，本研究对人髓系细胞系THP-1细胞的RNA基因表达谱展开分析。针对经三种细菌（B. fungorum、S. marcescens、P. myrsinacearum）来源脂多糖处理的THP-1细胞，本研究开展了RNA-seq转录组分析。实验整体设计：分别以B. fungorum、S. marcescens、P. myrsinacearum来源的脂多糖处理THP-1细胞，对其mRNA表达谱进行检测。