Tristetraprolin (TTP) regulated splenic transcriptome in "TTPΔARE" mice. Mus musculus

Tristetraprolin (TTP) binds to specific AU-rich elements in the 3'UTR of certain transcripts and regulates post-transcriptional gene expression by increasing the rate of mRNA turnover. Here, we generated a novel TTP mouse line (TTPΔARE) with a 136 base deletion in the 3'UTR AU-rich region of TTP mRNA, which resulted in a moderate increase in TTP protein expression in many tissues. In this study, we evaluated the effects of the moderate TTP overexpression on the overall pattern of gene expression in spleen, to investigate the effect of this mutation on mRNA targets of TTP under normal conditions. We utilized "TTPΔARE" (136 b deletion in the 3'UTR AU-rich region of Zfp36 mRNA) mice that overexpress TTP and compared the transcriptomic changes to the littermate "WT" mice. Spleen mRNA from four WT and four "TTPΔARE" mice were subjected to mRNA-Seq. All the animals used in this study were males between the ages of 8-12 weeks and were on a C57BL/6 background. Overall design: Examination of gene expression differences in mouse spleen from 4 wild-type and 4 TTPΔARE male mice aged 8-12 weeks

三联体脯氨酸富含域蛋白（Tristetraprolin, TTP）可结合特定转录本3'非翻译区（3' untranslated region, 3'UTR）内的富AU元件（AU-rich element, ARE），通过加快mRNA周转速率调控转录后基因表达。本研究构建了一种新型TTP小鼠品系（TTPΔARE），其Zfp36 mRNA的3'UTR富AU区域存在136个碱基缺失，该突变使多个组织中的TTP蛋白表达量适度升高。本研究旨在评估TTP适度过表达对小鼠脾脏基因表达整体模式的影响，以探究该突变在正常生理条件下对TTP mRNA靶标的调控作用。我们选用过表达TTP的"TTPΔARE"小鼠（Zfp36 mRNA的3'UTR富AU区域存在136 bp缺失），并将其转录组变化与同窝野生型（WT）小鼠进行对比。采集4只WT小鼠与4只"TTPΔARE"小鼠的脾脏mRNA，开展mRNA测序（mRNA-Seq）。本研究所用实验动物均为8~12周龄的雄性C57BL/6背景小鼠。实验整体设计为：对8~12周龄雄性C57BL/6背景的4只野生型与4只"TTPΔARE"小鼠的脾脏组织进行基因表达差异分析。