Additional file 2: Figures S1–S4. of The midgut transcriptome of Aedes aegypti fed with saline or protein meals containing chikungunya virus reveals genes potentially involved in viral midgut escape

Figure S1 Shared DE genes between CHIKV infected and non-infected SM/PM samples at 1 and 2 days post-feeding/infection (dpf/pi). A Venn diagram. B Description of shared DE genes. Figure S2 Median logFC values for upregulated and downregulated DE genes in response to CHIKV infection and SM/PM feeding at 1 and 2 days post-feeding/post-infection (dpf/pi). logFC, logarithmic fold-change. Figure S3 Median-normalized expression levels (at FPKM) of immunity related genes (A), RNAi pathway genes (B), and apoptotic pathway genes (C). Data show a comparison between sugarfed controls and SM/PM RNA-Seq libraries at 1 and 2 days post-feeding/post-infection (dpf/pi) and are presented as heatmaps for each transcript (vertical axis) at each time point (horizontal axis), with yellow and blue indicating high and low levels of expression, respectively. Figure S4 Expression profiles of two putative serine collagenase genes in response to CHIKV infection and SM/PM/BM ingestion in midguts at 1, 2 and 4 days post-feeding/post-infection (dpf/pi). qRT-PCR was performed using total RNA extracted from midguts of mosquitoes, which had received a CHIKV containing or virus-free BM/PM/SM at 1, 2, and 4 dpf/pi. Midguts of sugarfed mosquitoes were used as control. Mean values with standard deviation (SD) from three independent experiments are shown. Significances between sugarfed and other samples were determined by Student t test (* at P ≤ 0.05, ** at p ≤ 0.01). AeLT, late trypsin, AAEL013284; AeSP1, putative serine collagenase 1 precursor, AAEL007432. (ZIP 14704 kb)

补充图S1：基孔肯雅病毒（CHIKV, Chikungunya virus）感染与未感染的SM/PM样本在取食/感染后1、2天的共享差异表达基因（DE genes, differentially expressed genes）。A为维恩图（Venn diagram），B为共享差异表达基因的说明。补充图S2：基孔肯雅病毒感染与SM/PM取食后1、2天的上调与下调差异表达基因的中位数对数倍变值（logFC, logarithmic fold-change，对数倍变化）。补充图S3：免疫相关基因（A）、RNAi通路基因（B）以及凋亡通路基因（C）的中位数标准化表达水平（以FPKM计，FPKM, Fragments Per Kilobase of transcript per Million mapped reads）。数据对比了蔗糖饲喂对照组与SM/PM的RNA测序文库在取食/感染后1、2天的样本，并以热图展示每个转录本（纵轴）在各时间点（横轴）的表达情况，黄色与蓝色分别代表高、低表达水平。补充图S4：两个推定的丝氨酸胶原酶基因在基孔肯雅病毒感染以及SM/PM/BM取食后1、2、4天的中肠内的表达谱。实验采用从蚊子中肠提取的总RNA进行实时定量聚合酶链反应（qRT-PCR, quantitative real-time polymerase chain reaction），这些蚊子分别接受了含基孔肯雅病毒或无病毒的BM/PM/SM处理，并在取食/感染后1、2、4天取样。以蔗糖饲喂的蚊子中肠作为对照。结果展示了3次独立实验的平均值与标准差（SD）。采用学生t检验比较蔗糖饲喂组与其他组的显著性差异（*表示P ≤ 0.05，**表示P ≤ 0.01）。AeLT为晚期胰蛋白酶（late trypsin），基因编号AAEL013284；AeSP1为推定的丝氨酸胶原酶1前体，基因编号AAEL007432。（压缩包大小14704 kb）