Additional file 2 of An integrated QTL and RNA-seq analysis revealed new petal morphology loci in Brassica napus L.
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Additional file 2: Table S1. The statistical data of MPA, MPP, PAR, MPL, MPW, and PCD in the KN DH population under six microenvironments, including 17WH, 17YL, 18WH, 18YL, 19WH, and 19YL. Table S2. The Pearson correlation coefficient of MPA, MPP, PAR, MPL, MPW, and PCD in 17WH. The p-value was represented by “*” (p < 0.05, significant) and “**” (p < 0.01, highly significant). Table S3. The Pearson correlation coefficient of MPA, MPP, PAR, MPL, MPW, and PCD in 17YL. The p-value was represented by “*” (p < 0.05, significant) and “**” (p < 0.01, highly significant). Table S4. The Pearson correlation coefficient of MPA, MPP, PAR, MPL, MPW, and PCD in 18WH. The p-value was represented by “*” (p < 0.05, significant) and “**” (p < 0.01, highly significant). Table S5. The Pearson correlation coefficient of MPA, MPP, PAR, MPL, MPW, and PCD in 18YL. The p-value was represented by “*” (p < 0.05, significant) and “**” (p < 0.01, highly significant). Table S6. The Pearson correlation coefficient of MPA, MPP, PAR, MPL, MPW, and PCD in 19WH. The p-value was represented by “*” (p < 0.05, significant) and “**” (p < 0.01, highly significant). Table S7. The Pearson correlation coefficient of MPA, MPP, PAR, MPL, MPW, and PCD in 19YL. The p-value was represented by “*” (p < 0.05, significant) and “**” (p < 0.01, highly significant). Table S8. The detailed information of all identified QTLs detected in the present study. The “position”, “from”, and “to” were presented by the genetic distance. Table S9. The detailed information of all consensus QTLs detected in the present study, and the “position”, “from”, and “to” were presented by the genetic distance. Table S10. The detailed information of all unique QTLs detected in the present study, and the “position”, “from”, and “to” were presented by the genetic distance. Table S11. All PDRs collected in the present study. Table S12. The detailed information of each RNA-seq sample. Table S13. The primers used in the present study. Table S14. The detailed information of candidate genes and high-confidence candidate genes. Table S15. The sequence variations of candidate genes. Table S16. The expression profile (presented by FPKM) of 152 candidate PDRs expressed in the flower stage petals (B ~ J) and their log2(FC) between different petal samples (K ~ P). The zero values of FPKMs were replaced by 0.01 to avoid invalid parameters. Table S17. The expression profile (presented by FPKM) of 168 candidate PDRs expressed in the bud stage petals (B ~ J) and their log2(FC) between different petal samples (K ~ P). The zero values of FPKMs were replaced by 0.01 to avoid invalid parameters. Table S18. The previously reported petal size QTLs of B. napus that are consistent with the petal morphology QTLs detected in the present study.
附加文件2:表S1。KN DH群体在6个微环境(17WH、17YL、18WH、18YL、19WH、19YL)下的MPA、MPP、PAR、MPL、MPW及PCD统计数据。
表S2。17WH环境下MPA、MPP、PAR、MPL、MPW及PCD的皮尔逊相关系数。P值以"*"表示(p < 0.05,差异显著),"**"表示(p < 0.01,差异极显著)。
表S3。17YL环境下MPA、MPP、PAR、MPL、MPW及PCD的皮尔逊相关系数。P值以"*"表示(p < 0.05,差异显著),"**"表示(p < 0.01,差异极显著)。
表S4。18WH环境下MPA、MPP、PAR、MPL、MPW及PCD的皮尔逊相关系数。P值以"*"表示(p < 0.05,差异显著),"**"表示(p < 0.01,差异极显著)。
表S5。18YL环境下MPA、MPP、PAR、MPL、MPW及PCD的皮尔逊相关系数。P值以"*"表示(p < 0.05,差异显著),"**"表示(p < 0.01,差异极显著)。
表S6。19WH环境下MPA、MPP、PAR、MPL、MPW及PCD的皮尔逊相关系数。P值以"*"表示(p < 0.05,差异显著),"**"表示(p < 0.01,差异极显著)。
表S7。19YL环境下MPA、MPP、PAR、MPL、MPW及PCD的皮尔逊相关系数。P值以"*"表示(p < 0.05,差异显著),"**"表示(p < 0.01,差异极显著)。
表S8。本研究中检测到的所有已鉴定数量性状位点(Quantitative Trait Locus,QTL)的详细信息。"位置""起始"及"终止"以遗传距离表示。
表S9。本研究中检测到的所有一致性QTL的详细信息,"位置""起始"及"终止"以遗传距离表示。
表S10。本研究中检测到的所有独特QTL的详细信息,"位置""起始"及"终止"以遗传距离表示。
表S11。本研究中收集的所有PDRs的相关信息。
表S12。本研究中每个RNA测序(RNA-seq)样本的详细信息。
表S13。本研究中使用的引物信息。
表S14。候选基因及高可信度候选基因的详细信息。
表S15。候选基因的序列变异信息。
表S16。152个在花期花瓣(B~J)中表达的候选PDR的表达谱(以FPKM表示),以及不同花瓣样本间的log₂(FC)值(K~P)。为避免生成无效参数,将FPKM值中的零替换为0.01。
表S17。168个在蕾期花瓣(B~J)中表达的候选PDR的表达谱(以FPKM表示),以及不同花瓣样本间的log₂(FC)值(K~P)。为避免生成无效参数,将FPKM值中的零替换为0.01。
表S18。本研究检测到的花瓣形态QTL与此前报道的甘蓝型油菜(Brassica napus,B. napus)花瓣大小QTL相一致的相关信息。
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figshare
创建时间:
2024-08-15



