Specificity of the real-time PCR assay for the detection of D. suzukii.

All samples were run in duplicate, therefore mean Cq and mean Melt Peak Temperatures are provided. Positive identifications depend on the amplification as well as melt peak temperatures, with D. suzukii melt peaks = 73°C (−0.2/+0.4°C). Further confirmation of the identifications was obtained via analysis of the difference plot of the melt curves.1All species were first identified morphologically by Dr Disna Gunawardana (or Dr Gunawardana confirmed identifications by the submitters), and subsequently confirmed also via sequencing the COI gene. See footnote of Table 2 for further details on identifiers/submitters for the samples.§Amplification after the cut-off Cq value.

所有样本均进行双份重复实验，故本数据集给出平均循环阈值（Cycle threshold, Cq）及平均熔解峰温度（Melt Peak Temperatures）。阳性鉴定结果需同时结合扩增情况与熔解峰温度进行判定，其中斑翅果蝇（Drosophila suzukii）的熔解峰温度为73°C（偏差范围−0.2/+0.4°C）。通过熔解曲线差异图分析，可对鉴定结果进行进一步验证¹。所有物种首先由迪斯纳·古纳瓦德纳博士（Dr. Disna Gunawardana）完成形态学鉴定（或由样本提交者自行鉴定并经古纳瓦德纳博士确认），后续通过细胞色素C氧化酶亚基I（Cytochrome C Oxidase Subunit I, COI）测序完成再次验证。有关样本标识者与提交者的详细信息，请参见表2的脚注。§ 循环阈值（Cq）截值之后的扩增反应。