Agro_AD1484

Agro_AD1484 (AD1484:A348dB/pAD2079,virB on a multicopy plasmid). Add 200 ul of induction media to one of the induction plate (containing lawn of Agrobacterium AD1484) and wash the plate by gently rolling the liquid. Take this liquid and add to the other plate. Repeat the same. This should get enough cells dissolved in the liquid.Add gold to this: 1ul of gold to 4 ul of cells.

Agro_AD1484（AD1484:A348dB/pAD2079，virB基因位于多拷贝质粒上）。向一块涂布有农杆菌AD1484菌苔的诱导平板中加入200微升诱导培养基，轻轻晃动液体以冲洗平板。取该液体转移至另一块平板并重复上述操作，此步骤可获得足够量的溶解于液体中的菌体细胞。向所得细胞悬液中加入金颗粒：每4微升细胞液添加1微升金颗粒。