Epigenetic landscape of Human Brains by Single Nucleus DNA Methylation and Chromatin Conformation Profiling - pool43_h1930002_CaB. Epigenetic landscape of Human Brains by Single Nucleus DNA Methylation and Chromatin Conformation Profiling - pool43_h1930002_CaB

Illustrating the cellular architecture of the human brain is critical to understanding its diverse functions and complex animal behaviors. Single nucleus methylation sequencing was applied to regions of human brains. we identified distinct cell clusters and formed them in a hierarchical taxonomy. These clusters include known primary brain cell types and possible sub-types. We use these data to identify the epigenomic characteristics and to define specific regulatory elements for each cell cluster. Overall design: Apply snmC-seq3 to brain regions to identify epigenomic landcape of the adult human brains

阐明人类大脑的细胞架构，对于理解其多样功能与复杂的动物行为至关重要。我们对人类大脑的多个脑区实施了单细胞核甲基化测序（single nucleus methylation sequencing），成功识别出多组独特的细胞簇，并构建了其层级分类体系。这些细胞簇涵盖了已被广泛认知的主要脑内细胞类型，以及潜在的细胞亚型。我们依托本数据集解析了各组细胞簇的表观基因组特征（epigenomic characteristics），并明确了每一类细胞簇的特异性调控元件。
实验整体设计：采用snmC-seq3对脑区进行测序，以解析成人大脑的表观基因组全景（epigenomic landscape）。